I don't know what I did wrong? Help. Here is what I have: -I have isolated a p53 mutant (Ser->Ala) in which Serene residue is replaced by Alanine. -Q: Assuming that this Serene residue in normal p53 makes 2 hydrogen bonds with DNA, and that each hydrogen bond is worth 2 Kcal/mole, calculate the equilibrium constants you would expect the mutant protein to have specific and non-binding DNA.. My guess- This replacement does affect the overall stability. So Serene is polar, hydrophilic while Alanine is hydrophobic. -A: for Kb specific I found it to be 1.27x10^11 M^-1 ( I have correct, I think) -----I can't get Kb non-specific right Here is my answer: delta G= -0.6 ln (10^7)= -9.67+4 = -5.67 -5.67= -0.6 ln k eq 9.45= ln k eq (=> (or goes to)) e^9.45= k eq Then I have... k eq=1.27x10^4 M^-1 EDIT: Nevermind. Found the answer from a friend's help. Mods can close this thread.
